Figure 5.

Spontaneous Assemblies Are Not Driven by Retino-Tectal Inputs

(A) Example of a spontaneously emerging retinotopic-like map 24 hr after retinal-input deprivation (RID) of the left tectum (right eye enucleation at 7 dpf). Spontaneous assemblies whose centroids lay on the normal and RID tectum are shown on the left and right panels, respectively. Data are represented as in Figure 4G.

(B) Normalized topography of spontaneous assemblies for normal (top) and RID (bottom) tecti (n = 5). Data are represented as in Figure 4F.

(C) Density plot of the normalized rostro-caudal position of each neuron against the normalized rostro-caudal centroid position of its spontaneous assemblies for both the normal (left) and RID tecti (middle) and for assemblies’ null models (right). Grayscale: data density.

(D) Regression of the data shown in (C) for the normal (green) and RID (red) tecti (mean and 95% confidence interval). The dashed black line shows the regression result for null models.

(E) Relative area as a function of compactness index (see Supplemental Experimental Procedures) for spontaneous assemblies in the normal (green) and RID (red) tecti. Only significantly compact assemblies are shown (significance threshold indicated by dashed line). The black solid line outlines the region occupied by 99% of the normal tecti spontaneous assemblies with random 25% rearrangement of their neuronal composition. Spontaneous assemblies in RID tecti are not significantly different from this rearranged assembly population.