Table 1. Strains and plasmids used in this study.
| Name | Description | Referenz |
|---|---|---|
| BZB 1011 E2C | pColE2-P9 | [17] |
| BZB 1011 | Same as BZB 1011 E2C without the colicin plasmid | [17] |
| EMO3-C | BZB 1011 E2C pMO3 | This study |
| EMO3-S | BZB 1011 pMO3 | This study |
| pEX-K-Colicin | Commercially obtained vector pEX-K-Colicin (MWG Eurofins, Ebersberg) harboring the complete Colicin E2 operon with the exception of cea. Slight sequence changes have been incorporated to allow introduction of necessary restriction sites. | This study |
| pColE2-P9 | Native colicin plasmid with complete Colicin E2 operon comprising the cea, cei and cel gene | [9] |
| pKES258 | mVenus | unpublished |
| pKEHB1 | mCerulean (A206K) | unpublished |
| pMO1 | Ligation of ClaI—HindIII fragment of pEX-K-Colicin with the backbone of pBAD24GFP | This study |
| pMO2 | Ligation of pMO1 with a mVenus PCR fragment (restriction sites EcoRI and SacI) of pKES258 | This study |
| pMO3 | Exchange of cel gene in pMO2 with a CFP PCR fragment (restriction sites BamHI and AgeI) of pKEHB1 | This study |