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. 2015 Feb 9;9(4):1223–1228. doi: 10.3892/etm.2015.2275

Table IV.

Materials added to the centrifuge tube.

Component Dosage (μl)
Reaction solution (Table III) 12
5X reverse transcription buffer 4
dNTP mixture (10 mmol/l each) 2
RNase Inhibitor (10 U/μl) 1
ReverTra Ace 1