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SP does not induce the proliferation of ST2 cells. Percentages of BrdU positive cells 48 h after SP treatment under (A) normal serum conditions or (B) serum-free conditions. Cells were treated with 20 μM BrdU for the final 6 h. The percentages of BrdU positive cells are presented as the mean ± standard deviation (n=3 per group). Con, control; SP, substance P; BrdU, 5-bromo-2′-deoxyuridine.
