Table I.
Target gene | Primer sequences | Amplification length (bp) |
---|---|---|
Perforin | Forward 5′-CAGGTCAACATAGGCATCCACG-3′ Reverse 5′-GAACAGCAGGTCGTTAATGGAG-3′ |
160 |
Granzyme B | Forward 5′-GAAACGCTACTAACTACAGG-3′ Reverse 5′-CCACTCAGCTAAGAGGT-3′ |
126 |
INF-γ | Forward 5′-GCAGAGCCAAATTGTCTCCT-3′ Reverse 5′-ATGCTCTTCGACCTCGAAAC-3′ |
290 |
TNF-α | Forward 5′-CGAGTGACAAGCCTGTAGC-3′ Reverse 5′-CCTTCTCCAGCTGGAAGAC-3′ |
363 |
TNF-β | Forward 5′-AGGCATGAGGGATCACAG-3′ Reverse 5′-AAAGAGGTTTATTGGGCTTC-3′ |
115 |
FasL | Forward 5′-TGTTTATGAGCCAGACAAATGG-3′ Reverse 5′-AAGACAGTCCCCCTTGAGGT-3′ |
203 |
INF, interferon; TNF, tumor necrosis factor; FasL, Fas ligand.