Table I.
Primer sequences for the amplified genes.
| Gene name | Forward (5′-3′) | Reverse (3′-5′) | Length (bp) |
|---|---|---|---|
| SERCA2a | TCTGACTTTCGTTGGCTGTG | GCCTTTGTTATCCCCAGTGA | 121 |
| ANP | ATCTGATGGATTTCAAGAACC | CTCTGAGACGGGTTGACTTC | 169 |
| BNP | ACAATCCACGATGCAGAAGCT | GGGCCTTGGTCCTTTGAGA | 91 |
| MMP-2 | CTGGGCAACAAGTATGAGAG | GTGTAGGTGTAGATAGGGGC | 218 |
| TIMP-2 | GCATCACCCAGAAGAAGAGC | GTTTCCAGGAAGGGATGTCA | 338 |
| GSK-3β | ATGGCAGCAAGGTAACCACAG | TCTCGGTTCTTAAATCGCTTGTC | 193 |
| GAPDH (reference gene) | TGCTGAGTATGTCGTGGAGT | GTCTTCTGAGTGGCAGTGAT | 288 |
SERCA2a, sarcoendoplasmic reticulum Ca2+-ATPase 2a; ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; MMP-2, matrix metalloproteinase-2; TIMP-2, tissue inhibitor of MMP-2; GSK-3β, glycogen synthase kinase-3β; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.