Table 2.
Statistical evaluation of nuclear and cytosol staining between different time points
| A | Pre EE2 (n=10) mean (S.E.) | During + Post EE2 (n=8) mean (S.E.) | P-value | B | Pre EE2 (n=10) mean (S.E.) | During EE2 (n=3) mean (S.E.) | Post EE2 (N=5) mean (S.E.) | P-value | ||
|---|---|---|---|---|---|---|---|---|---|---|
| ER | nuc | 166.7 (9.0) | 68.3 (18.2) | 0.0006** | ER | nuc | 166.7 (9.0) | 68.0 (28.8) | 68.6 (26.1) | 0.0024** |
| PgR | nuc | 19.2 (15.8) | 113.1 (24.1) | 0.01* | PgR | nuc | 19.2 (15.8) | 171.3 (17.9) | 78.2 (26.8) | 0.0113* |
| Ki67 | nuc | 12.3 (1.96) | 22.5 (5.82) | 0.2 | Ki67 | nuc | 12.3 (1.96) | 10.0 (5.0) | 30.0 (7.07) | 0.052 |
| BRCA1 | nuc | 96.6 (5.79) | 64.8 (16.7) | 0.3 | BRCA1 | nuc | 96.6 (5.79) | 57.3 (34.3) | 69.4 (20.3) | 0.56 |
| cyto | 0 (0) | 36.6 (17.8) | 0,047* | cyto | 0 (0) | 0 (0) | 58.6 (23.9) | 0.0126* | ||
| AR | nuc | 119.2 (9.79) | 44.2 (17.2) | 0.039* | AR | nuc | 119.2 (.979) | 75.3 (28.7) | 25.6 (18.7) | 0.0065** |
| cyto | 14.0 (9.79) | 68.75 (15.7) | 0.0097** | cyto | 14.0 (9.79) | 98.0 (1.52) | 51.2 (22.0) | 0.0092** | ||
| PI3K | cyto | 83.3 (9.39) | 95.0 (5.73) | 0.31 | PI3K | cyto | 83.3 (9.3) | 98.3 (1.66) | 93.0 (9.41) | 0.53 |
| AKT | nuc | 44.3 (11.3) | 37.2 (12.6) | 0.72 | AKT | nuc | 44.3 (11.3) | 35.6 (25.0) | 38.2 (16.0) | 0.9 |
| cyto | 75.2 (9.69) | 76.8 (11.8) | 0.58 | cyto | 75.2 (9.69) | 83.3 (12.0) | 73.0 (18.3) | 0.81 | ||
| pAKT | nuc | 7.2 (4.5) | 17.2 (16.5) | 0.63 | pAKT | nuc | 7.2 (4.5) | 0 (0) | 27.6 (26.3) | 0.44 |
| cyto | 25.9 (11.4) | 23.1 (15.1) | 0.57 | cyto | 25.9 (11.4) | 0 (0) | 37.0 (22.6) | 0.36 | ||
| IGT1Rb | cyto | 52.5 (13.3) | 32.5 (16.1) | 0.37 | IGT1Rb | cyto | 52.5 (13.3) | 30.0 (30.0) | 34.0 (21.4) | 0.64 |
| TUNEL | 5.1 (2.42) | 3.0 (1.26) | 0.74 | TUNEL | 5.1 (2.44) | 2.0 (2.0) | 3.6 (1.74) | 0.75 | ||
The Wilcoxon test/the Kruskal-Wallis test was used to analyze the significance of differences between groups.
nuc: nuclear, cyto: cytosol.
*<0.05, **<0.01.