Fig. 6.

The effect of salsolinol on glutamate-induced loss of mitochondrial membrane potential in mouse hippocampal cultures. Primary hippocampal cultures were treated with glutamic acid (1 mM) or salsolinol (50 or 500 µM) alone or in combination, for 6 h. The mitochondrial membrane potential was detected with JC-1 Assay Kit. The results were normalized to the fluorescence in vehicle-treated cells and expressed as red to green fluorescence ratio. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicantes in each experimental ranged from 5 to 8. ***P < 0.001 versus control cultures; ^# P < 0.05, ^## P < 0.01 versus the cultures exposed to glutamic acid