Specificity of PCR for donkey, horse, cattle, and pig DNA with each of their specific primers. a Donkey-specific PCR. b Horse-specific PCR. c Cattle-specific PCR. d Pig-specific PCR. Lane 1 is a no-template control, and lanes 2–5 show the DNA from donkey, horse, cattle, and pig samples, respectively. M, 20-bp ladder marker