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. 2015 Feb 3;7(2):522–542. doi: 10.3390/v7020522

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Expression of gB in virus-infected cells. Cell lysates were prepared from infected equine dermal (ED) cells and proteins were separated under reducing conditions by sodium dodecyl sulfate (SDS)-10%-polyacrylamide gel electrophoresis (PAGE). The blots were incubated with anti-gB monoclonal antibody (MAb)-3F6 and bound antibody detected with anti-mouse IgG peroxidase conjugate. EHV-1, EHV-4 and related recombinant virus proteins with an apparent molecular weight of approximately 130 and 76 kD were detected that are not present in mock-infected cells. Rabbit anti-β-actin antibody was used as a loading control (molecular weight of approximately 42 kDa).