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. 2015 Feb 3;7(2):522–542. doi: 10.3390/v7020522

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The role of gB in EHV-1 cellular tropism. Chinese hamster ovary (CHO)-K1, CHO-A, CHO-B, CHO-C, Crandell feline kidney (CrFK), Madin-Darby canine kidney (MDCK), Rabbit kidney (RK13) and Vero cells were infected at an MOI of 0.1 with the parental EHV-1 and EHV-1_gB4, all of which express eGFP. At 24 h p.i., cells were inspected with a fluorescent microscope (Zeiss Axiovert, Jena, Germany) and images were taken with a CCD camera (Zeiss Axiocam, Jena, Germany). The bar represents 100 μm and the white frames contain magnified inserts of the selected areas. CHO-K1, CHO-A, CHO-B and CHO-C, and RK13 cells were highly resistant and MDCK virtually resistant to parental and recombinant EHV-4 infection. In addition, CrFK were highly resistant and Vero cells virtually resistant to parental and recombinant EHV-1 infection.

The role of gB in EHV-1 cellular tropism. Chinese hamster ovary (CHO)-K1, CHO-A, CHO-B, CHO-C, Crandell feline kidney (CrFK), Madin-Darby canine kidney (MDCK), Rabbit kidney (RK13) and Vero cells were infected at an MOI of 0.1 with the parental EHV-1 and EHV-1_gB4, all of which express eGFP. At 24 h p.i., cells were inspected with a fluorescent microscope (Zeiss Axiovert, Jena, Germany) and images were taken with a CCD camera (Zeiss Axiocam, Jena, Germany). The bar represents 100 μm and the white frames contain magnified inserts of the selected areas. CHO-K1, CHO-A, CHO-B and CHO-C, and RK13 cells were highly resistant and MDCK virtually resistant to parental and recombinant EHV-4 infection. In addition, CrFK were highly resistant and Vero cells virtually resistant to parental and recombinant EHV-1 infection.