Figure 1.
Effect of pooled (n = 4) class III Hantavirus Cardiopulmonary Syndrome (HCPS) patient plasma on cell barrier properties of Vero E6 cells measured by Electric Cell-substrate Impedance Sensing (ECIS). (A) Vero E6 cells were plated in electrode-containing dishes at 105 cells/cm2 and allowed to attach, spread, and organize for at least 20 h. Cellular impedance was measured continuously at a single frequency of 4000 Hz. Increase in resistance corresponds to increasing cell barrier function. a: The rapid increase in resistance at about 20 h occurred after the media was exchanged; b: Class III plasma collected on day of admission and healthy control plasma were added to monolayers; (B) Comparing the effect of patient plasma and controls by normalizing data from (A), at baseline established after media was refreshed. Error bars represent duplicate measurements; (C) Effect of 4-fold dilution on the barrier function of confluent monolayers of pooled plasma from classes I, and III patients. Data also show that the loss of resistance is caused by a concentration-dependent fast acting component and a slowly acting component, which is relatively insensitive to dilution.