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. 2015 Feb 10;7(2):559–589. doi: 10.3390/v7020559

Figure 5.

Figure 5

SNV induces loss of cell barrier function in intact tight-junction forming monolayers of polarized Vero and TIVE. (A) Confocal images of tight junctions in polarized monolayers; (B) Development of tight junctions in polarized monolayers grown in 6 mm microporous filter inserts at 150,000 cells/well was measured on days shown in the plot of TER versus time for HLMVEC, TIVE and Vero E6 cells. The error bars are standard deviations from 12 monolayers; (C, D) Effect of live SNV on the barrier integrity of Vero E6 and TIVE monolayers under different conditions described in the text. Error bars represent standard deviations from independent replicates of 3 monolayers.