Table 1.
ORF57 cofactors.
| ORF57 cofactor | Cofactor function | Interaction region in ORF57 (aa) | Interaction region in cofactor (aa/Domain) | Proved direct interaction | Proposed function of interaction | Conserved interaction in homologues | Citation | |
|---|---|---|---|---|---|---|---|---|
| Cellular cofactors | ||||||||
| Aly/REF | RNA-binding protein | 1–251 (181–215) | 103–163 (RRM) | + | RNA stability/Export (?) | + | [16,36] | |
| CBP80 | RNA-binding protein | ND | ND | − | RNA stability/Export (?)/Translation | − | [45,56] | |
| CKII α | Protein kinase | 181–455 | ND | + | Phopshorylation | + | [105] | |
| CKII α' | Protein kinase | 387–455 | ND | + | Phopshorylation | + | [105] | |
| CKII β | Protein kinase | 181–215 | 150-182 | + | Phopshorylation | + | [105] | |
| hnRNP K | RNA-binding protein | 17–181/329–387 | 240–337 (KI) interactive domain | + | ? | + | [105] | |
| hnRNP U | RNA-binding protein | 1–251 | ND | − | ? | − | [90] | |
| Nup62 | Nucleoporin | ND | ND | − | ? | + | [106] | |
| NXF1 | RNA export factor | ND | ND | − | Export (?) | + | [56] | |
| OTT3 | RNA-binding protein | 1–251 | 488–890 (SPOC) | + | RNA stability/Polyadenylation | + | [38] | |
| PABPC1 | RNA-binding protein | 1–251 | 1–370 (RRM1-4) | + | RNA stability | − | [56] | |
| PCBP1 | RNA-binding protein | 179–205 | 48–96 (KH-I) | + | Translation | − | [74] | |
| PYM | Scaffold protein | ND | ND | − | Translation | − | [75] | |
| RBM15 | RNA-binding protein | 1–251 | 530–977 (SPOC) | + | RNA stability/Polyadenylation | + | [38] | |
| SRSF1 | RNA-binding protein | ND | ND | − | RNA splicing | + | [20] | |
| SRSF3 | RNA-binding protein | 1–251 | 1–83 (RRM) | − | RNA splicing | + | [31] | |
| U snRNPs | Splicing factors | 1–251 | ND | − | RNA splicing | + | [20] | |
| U2AF35 | RNA-binding protein | 1–251 | ND | − | RNA splicing | + | [20] | |
| UAP56 | RNA-binding protein | ND | ND | − | Export (?) | + | [45] | |
| UBE2O | Ubiqitin-conjugating enzyme E2O | 1-251 | ND | - | ? | - | [90] | |
| UIF | RNA-binding protein | ND | ND | + | Export (?) | − | [50] | |
| Viral cofactors | ||||||||
| K-bZIP | Transcription factor | 96–215 | ND | + | ? | − | [85,86] | |
| ORF23 | ? | ND | ND | + | ? | − | [86] | |
| ORF50 | Transcription factor | ND | 1–518 (E3-LR) | + | Transactivation (?) | − | [83,84,86] | |
| ORF52 | ? | ND | ND | + | ? | − | [86] | |
| ORF57 | Posttranscriptional regulator | 251–455 | 251–455 | + | Self-interaction | + | [86,90,105] | |
| ORF61 | Ribonucleotide reductase | ND | ND | + | ? | + | [86] | |
| ORF63 | Tegument | ND | ND | + | ? | − | [86] | |
| ORF67.5 | ? | ND | ND | + | ? | + | [86] | |
| ORF68 | Glycoprotein | ND | ND | + | ? | + | [86] | |
Cellular and KSHV (viral) proteins found to interact with ORF57 are summarized in this table. An interaction (+) is considered direct only if it has been proved with a GST pulldown assay of the two purified proteins or by using yeast two-hybrid systems. If a cofactor was found to interact with at least one ORF57 homologue, the interaction was considered being conserved. ND—not determined, RRM—RNA-recognition motif, KI—hnRNP K interaction domain, KH-I—K homology domain type I, SPOC—spen paralogue and orthologue C-terminal domain, E3—ubiquitin ligase 3 domain, LR—leucine-rich domain. ?—unknown.