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. 2015 Feb 16;7(2):751–780. doi: 10.3390/v7020751

Table 3.

Comparison of clinically used viral diagnostics tests.

Virus Method Detection limit Advantages Disadvantages Refs.
Influenza isolation and identification of the virus 1 EID50/mL sensitivity time consuming [47]
ELISA 1.0 ng rapid high rate of false
positive results
[48]
RT-PCR 0.0256 HAU specificity
sensitivity
expensive
complicated, highly skilled stuff
[49]
qRT-PCR 10 copies /reaction [50]
HBV ELISA 0.5 pg/mL as presented above [51]
qRT-PCR 18 IU/mL [52]
HIV ELISA 0.9–1.2 IU/mL [53]
qPCR 18–65 copies/mL [54]