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. 2015 Jan 6;6:5849. doi: 10.1038/ncomms6849

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(a) AFM image of Rad4 with 514-bp undamaged DNA. The arrows indicate proteins bound on DNA. Scan area is 1 μm × 1 μm. (b) Zoomed images of examples of Rad4 bound on DNA. (c) Histogram of bend angle distribution for Rad4 bound to undamaged DNA. Total of 67 molecules were measured from three independent depositions. The solid-line curve represents fitting of these data to a Gaussian distribution in MATLAB, providing the centre of bend angle as 48.4° (±34.2°). The uncertainty in the bend angle is the sample s.d. derived from the Gaussian fit.