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. 2015 Jan 22;6:5874. doi: 10.1038/ncomms6874

Figure 5. Defective p53 response in human sebaceous tumours and SCs of mutant Lef1 mice.

Figure 5

(a–c) Immunofluorescent staining of p53 (red) and K14 (green) in SCC (n=2 mice, 3 tumours; a, arrows), sebaceous tumour of K15ΔNLef1 mice (n=4 mice, 10 tumours) (b) and human sebaceous tumour (c; n=7). (d–g) Immunofluorescent detection of p53 in HF bulge of wild-type (d,e) and K15ΔNLef1 mice (f,g) without (d,f) and following UV irradiation (e,g; n=3 mice). Nuclei stained in red (PI). (h) Quantification of p53 detection in HF of wild-type and K15ΔNLef1 mice following UV treatments (n=6 mice). Significance was calculated by Student’s t-test (***P<0.001) and s.d. was calculated. (i) Western blot analysis for p53, p-p53 and GapDH (loading control) in epidermal lysates from wild-type and K15ΔNLef1 epidermis 24 h following UV treatment (n=3 mice). (j) qRT–PCR analysis for p21 mRNA expression in unexposed and UV-treated samples of wild-type and K15ΔNLef1 mice (n=6 mice). Significance was calculated by Student’s t-test (***P<0.001). Scale bars, 200 μm.