Table 1. Tissue weights and blood biochemistry of ob/ob and ob/ob/Fsp27 −/− mice.
| Parameter |
ob/ob |
ob/ob/Fsp27−/− |
|||
|---|---|---|---|---|---|
| N | Mean±s.e.m. | N | Mean±s.e.m. | P-value | |
| Body weight (g) | 10 | 52.09±1.13 | 10 | 36.82±0.33 | <0.0001*** |
| Serum glycerol (mg ml−1) | 9 | 0.76±0.08 | 7 | 1.06±0.04 | 0.0092** |
| Serum NEFA (mEq l−1) | 7 | 1.19±0.15 | 9 | 1.15±0.10 | 0.8376 |
| Gonadal fat (g) | 9 | 2.08±0.19 | 8 | 0.18±0.01 | <0.0001*** |
| Subcuteneous fat (g) | 9 | 2.34±0.11 | 8 | 0.47±0.04 | <0.0001*** |
| Mesenteric fat (g) | 9 | 1.64±0.06 | 8 | 0.34±0.01 | <0.0001*** |
| Retroperitoneal fat (g) | 9 | 2.25±0.12 | 8 | 0.46±0.02 | <0.0001*** |
| Inguinal fat (g) | 9 | 0.50±0.05 | 8 | 0.08±0.01 | <0.0001*** |
| Muscle(gastrocnemius) (g) | 10 | 0.45±0.01 | 10 | 0.45±0.01 | 0.886 |
| Kidney (g) | 10 | 0.45±0.01 | 10 | 0.46±0.01 | 0.250 |
| Heart (g) | 10 | 0.16±0.002 | 10 | 0.16±0.003 | 0.307 |
| Spleen (g) | 10 | 0.10±0.006 | 10 | 0.11±0.003 | 0.398 |
| Liver (g) | 6 | 3.49±0.19 | 10 | 5.06±0.27 | <0.001*** |
| BAT (g) | 5 | 0.39±0.03 | 7 | 0.70±0.06 | <0.01** |
BAT, brown adipose tissue; N, the number of mice used; NEFA, non-esterified fatty acid.
Four-month-old chow fed ob/ob and ob/ob/Fsp27−/− mice were used. Quantitative data are presented as mean±s.e.m. Significance was established using a 2-tailed Student’s t-test. Differences were considered significant at P<0.05. **P<0.01, ***P<0.001.