Table 2. Body composition and tissue weights of BATless and BATless/Fsp27 −/− mice while on a HFD.
| Parameter |
BATless |
BATless/Fsp27−/− |
P value | ||
|---|---|---|---|---|---|
| N | Mean±s.e.m. | N | Mean±s.e.m. | ||
| Body weight (g) | 12 | 44.3±0.8 | 10 | 32.2±1.6 | <0.0001*** |
| Fat mass (g) | 12 | 17.9±0.5 | 10 | 7.0±2.3 | <0.0001*** |
| Lean mass (g) | 12 | 26.5±0.7 | 10 | 24.0±3.4 | 0.2272 |
| Liver (g) | 12 | 1.4±0.08 | 10 | 1.7±0.07 | 0.0071** |
| Gonadal fat (g) | 12 | 1.4±0.05 | 10 | 0.149±0.02 | <0.0001*** |
| BAT (mg) | 12 | 35.4±3.2 | 10 | 66.7±16.4 | 0.0445* |
| Heart (mg) | 12 | 136.4±3.0 | 10 | 144.6±4.0 | 0.1104 |
| Gastrocnemius (mg) | 12 | 160.9±2.8 | 10 | 172.1±4.2 | 0.0342* |
| Tibialis anterior (mg) | 12 | 45.6±2.6 | 10 | 57.1±1.5 | 0.0017** |
| Quadriceps (mg) | 12 | 148.5±7.3 | 10 | 183.5±9.2 | 0.0069** |
HFD, high fat diet; N, the number of mice used.
Three-month-old BATless and BATless/Fsp27−/− mice were challenged with a HFD (D12492, 60% kcal of fat) for 6 weeks. Quantitative data are presented as mean±s.e.m. Significance was established using a two-tailed Student’s t-test. Differences were considered significant at P<0.05. *P<0.05, **P<0.01, ***P<0.001.