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. 2015 Mar 10;2:10. doi: 10.3389/fmed.2015.00010

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Copyright © 2015 Müller-Krebs, Nissle, Tsobaneli, Zeier, Kihm, Kender, Fleming, Nawroth, Reiser and Schwenger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of podocin. Immunofluorescence staining of podocytes to investigate the effect of ±benfotiamine after 48 h of incubation on the localization of podocin in the control, glucose, and PDF group. Highest relocalization score was observable in the glucose and PDF group without benfotiamine. Representative images of podocin staining (Dylight488), upper panel, and a merge of podocin and nuclei (Hoechst), lower panel, scale bar represents 50 μm (A), quantitation of the relocalization of podocin (B). ^##P < 0.01 versus −benfotiamine, ^§P < 0.05 versus −benfotiamine. PDF, peritoneal dialysis fluid.