Skip to main content
. 2015 Mar 10;4(3):e231. doi: 10.1038/mtna.2015.5

Figure 4.

Figure 4

KRAS is a direct target of miR-4689 in mutant KRAS CRC cells. (a) Schematic of the human KRAS 3′-UTR; sequence alignment with miR-4689 shows complementary nucleotides for binding (dotted lines). (b) Luciferase activity of the KRAS-3′UTR reporter in the presence of 50 nM of miR-4689 or miR-NC. (c) Immunoblots of KRAS in SW480 KRASG12V and DLD1 KRASG13D cells treated with miR-NC or miR-4689. Actin-beta (ACTB) is the loading control. (d) qRT-PCR results show relative expression of KRAS mRNAs in SW480 KRASG12V and DLD1 KRASG13D cells treated with miR-NC or miR-4689. All data represent mean ± SEM.