Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 9;212(3):287–295. doi: 10.1084/jem.20142322

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Jay et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — TREM2 deficiency reduces astrocytosis and MAPT phosphorylation. (a and b) Astrocytosis was assessed in 4-mo-old APPPS1;Trem2^+/+ (a) and APPPS1;Trem2^−/− mice (b) using IHC for GFAP and 6E10 (n = 7–8). (c and f) The number of GFAP⁺ cells surrounding plaques was quantified (c; n = 3–4), and results were confirmed by qRT-PCR (f; n = 7–8). (d, e, g, and h) Hyperphosphorylated MAPT was detected in APPPS1;Trem2^−/− (e and h) and APPPS1;Trem2^+/+ mice (d and g) with AT8 (d and e) and AT180 antibodies (g and h; n = 7–8). Arrows indicate Congo red–positive plaques. (i) Quantification of the area of AT180 immunoreactivity revealed significant decreases in APPPS1;Trem2^−/− mice (n = 3–4). At least two independent experiments were performed for all analyses. Error bars indicate SEM. *, P < 0.05; **, P < 0.01. Bar, 50 µm.