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. 2015 Mar 9;212(3):297–306. doi: 10.1084/jem.20141499

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© 2015 Dobenecker et al.

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Figure 5. — The histone H3K27me3 demethylases Jmjd3 and Utx are required for iNKT cell generation. Thymi and spleens of mice with T cell–specific deficiency of Jmjd3 (Jmjd3^fl/fl; CD4-cre) or Utx (Utx^fl/fl; CD4-cre) were examined for the surface expression of CD4 and CD8 (A) and CD1d-Tet and TCRβ (B) by flow cytometry. Numbers indicate the percentage of cells in the corresponding gates. Representative dotplots from two independent experiments with more than three mice are shown. (C) Quantification of iNKT cell numbers in thymus and spleen of Jmjd3^fl/fl; CD4-cre, Utx^fl/fl; CD4-cre, and WT controls, from more than three independent experiments with three or more mice. Each dot represents a single mouse. Significance was determined by unpaired Student’s t test: *, P ≤ 0.05; **, P ≤ 0.01.