TABLE 1.
Locus specific information for PCR amplification and pyrosequencing.
| Locus (contig) | No. CpG sites | PCR primers (5′-3′) | Amplicon length (bp) | Annealing Temp (°C) | Sequencing primer (5′-3′) | Reference |
|---|---|---|---|---|---|---|
| H19 ICR (AF087017) | 6 | Outer reverse CTTAAATCCCAAACCATAACACTA | 217 | 61.5 | TGGTTGTAGTTGTGGAAT | Present study |
| Outer forward GTATATGGGTATTTTTTGGAGGT | ||||||
| Inner forward GTATATGGGTATTTTTTGGAGGT | ||||||
| Inner reverse Tag-ATATCCTATTCCCAAATAA | 53 | |||||
| IG-DMR (A117190) | 7 | Forward Tag-TTTATTGGGTTGGGTTTTGTTAG | 267 | 58 | Primer 1 CAATTACAATACCACAAAAT | Present study |
| 3 | Reverse AACCAATTACAATACCACAAAATT | Primer 2 CCATAAACAACTATAAACCT | Present study | |||
| KvDMR1 (U90095) | 7 | Forward TTAGTTTTTTGYGTGATGTGTTTATTA | 101 | 55 | TTGYGTGATGTGTTTATTA | Bourque et al. (2010) |
| Reverse Tag-CCCACAAACCTCCACACC | ||||||
| PEG3 DMR (AC006115) | 7 | Reverse Tag-CCTATAAACAACCCCACACCTATAC | 272 | 62 | GGGGGTAGTTGAGGTT | Boissonnas et al. (2010) |
| Forward TAATGAAAGTGTTTGAGATTTGTTG |
Tag-5′-biotin-GACGGGACACCGCTGATCGTTTA-3′—universal biotin labeled tag.