TABLE 5.
The estimated effect of 1 year of age on % methylation per CpG site per locus.
| Control group | FAS cases | Estimated difference in age effect on methylation between FAS and CON | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Locus | Site | Age effect | SE | P-value | Age effect | SE | P-value | FAS-CON | SE | P-value |
| H19 ICR | CpG1 | 0.02 | 0.16 | 0.890 | 0.03 | 0.10 | 0.758 | 0.01 | 0.19 | 0.962 |
| H19 ICR | CpG2 | 0.04 | 0.16 | 0.811 | 0.06 | 0.10 | 0.577 | 0.02 | 0.19 | 0.924 |
| H19 ICR | CpG3 | –0.16 | 0.16 | 0.298 | 0.02 | 0.10 | 0.851 | 0.18 | 0.19 | 0.326 |
| H19 ICR | CpG5 | 0.10 | 0.16 | 0.522 | –0.01 | 0.10 | 0.896 | –0.11 | 0.19 | 0.541 |
| H19 ICR | CpG6 | –0.09 | 0.16 | 0.546 | 0.08 | 0.10 | 0.448 | 0.17 | 0.19 | 0.360 |
| IG-DMR | CpG1 | –0.05 | 0.17 | 0.789 | –0.43 | 0.10 | <0.001 | –0.38 | 0.20 | 0.052 |
| IG-DMR | CpG2 | –0.19 | 0.17 | 0.273 | –0.72 | 0.10 | <0.001 | –0.53 | 0.20 | 0.007 |
| IG-DMR | CpG3 | 0.19 | 0.17 | 0.256 | –0.02 | 0.10 | 0.811 | –0.22 | 0.20 | 0.273 |
| IG-DMR | CpG4 | 0.17 | 0.17 | 0.311 | –0.17 | 0.10 | 0.089 | –0.35 | 0.20 | 0.081 |
| IG-DMR | CpG5 | –0.11 | 0.17 | 0.520 | –0.95 | 0.10 | <0.001 | –0.84 | 0.20 | <0.001 |
| IG-DMR | CpG6 | –0.02 | 0.17 | 0.922 | –0.45 | 0.10 | <0.001 | –0.43 | 0.20 | 0.030 |
| IG-DMR | CpG7 | 0.05 | 0.17 | 0.774 | –0.25 | 0.10 | 0.016 | –0.30 | 0.20 | 0.134 |
| IG-DMR | CpG8 | 0.03 | 0.17 | 0.878 | –0.44 | 0.10 | <0.001 | –0.47 | 0.20 | 0.019 |
| IG-DMR | CpG9 | –0.04 | 0.17 | 0.812 | –0.52 | 0.10 | <0.001 | –0.48 | 0.20 | 0.016 |
| IG-DMR | CpG10 | –0.03 | 0.17 | 0.863 | –0.40 | 0.10 | <0.001 | –0.37 | 0.20 | 0.059 |
| KvdMR | CpG1 | 0.26 | 0.17 | 0.122 | –0.11 | 0.10 | 0.255 | –0.37 | 0.20 | 0.056 |
| KvdMR | CpG2 | 0.11 | 0.17 | 0.513 | –0.04 | 0.10 | 0.687 | –0.15 | 0.20 | 0.443 |
| KvdMR | CpG3 | 0.10 | 0.17 | 0.562 | –0.14 | 0.10 | 0.148 | –0.24 | 0.20 | 0.218 |
| KvdMR | CpG4 | 0.14 | 0.17 | 0.412 | –0.09 | 0.10 | 0.363 | –0.23 | 0.20 | 0.243 |
| KvdMR | CpG5 | 0.26 | 0.17 | 0.126 | –0.03 | 0.10 | 0.769 | –0.29 | 0.20 | 0.142 |
| KvdMR | CpG6 | 0.18 | 0.17 | 0.284 | –0.22 | 0.10 | 0.023 | –0.40 | 0.20 | 0.038 |
| KvdMR | CpG7 | 0.15 | 0.17 | 0.380 | –0.12 | 0.10 | 0.225 | –0.27 | 0.20 | 0.170 |
| PEG3 DMR | CpG1 | –0.19 | 0.17 | 0.272 | 0.02 | 0.10 | 0.818 | 0.22 | 0.20 | 0.289 |
| PEG3 DMR | CpG2 | –0.28 | 0.17 | 0.115 | 0.25 | 0.10 | 0.017 | 0.53 | 0.20 | 0.010 |
| PEG3 DMR | CpG3 | –0.03 | 0.17 | 0.858 | 0.09 | 0.10 | 0.376 | 0.12 | 0.20 | 0.543 |
| PEG3 DMR | CpG4 | –0.11 | 0.17 | 0.523 | 0.03 | 0.10 | 0.793 | 0.14 | 0.20 | 0.495 |
| PEG3 DMR | CpG5 | –0.22 | 0.17 | 0.201 | –0.14 | 0.10 | 0.194 | 0.09 | 0.20 | 0.665 |
| PEG3 DMR | CpG6 | –0.19 | 0.17 | 0.284 | –0.16 | 0.10 | 0.125 | 0.03 | 0.20 | 0.895 |
| PEG3 DMR | CpG7 | –0.37 | 0.17 | 0.033 | 0.03 | 0.10 | 0.799 | 0.40 | 0.20 | 0.050 |
Effect is the estimated percentage difference in methylation between individuals of a specific age and those 1 year younger, in the specific group at the specific locus, using linear mixed-effects models, as described in methods section.