Figure 3.

Girinimbine inhibits morphogenesis of endothelial cells.

Notes: (A) Representative images of fluorescent stained HUVECs which were treated with different concentrations of girinimbine in comparison with untreated, positive (VEGF) and negative (sulforaphane) controls. (B) Quantification data for the effect of girinimbine on the tube formation indexes including percentage of covered area, total tube length, and number of connected tubes. Data are shown as means ± SEM of three independent experiments. Statistical significance is expressed as ***P<0.001; **P<0.01; *P<0.05 versus untreated control.

Abbreviations: HUVECs, human umbilical vein endothelial cells; SEM, standard error of the mean; VEGF, vascular endothelial growth factor.