Figure 7.

Girinimbine inhibits angiogenesis in the in vivo zebrafish embryo model. Notes: Zebrafish embryos at 24 hpf were dechorionated manually and treated with girinimbine (20 μg/mL), SU5416 (0.25 μg/mL), and 1% DMSO for 24 hours. Thereafter, the treatment was removed and the embryos were maintained in normal embryo water for another 24 hours and euthanized, then fixed in 4% paraformaldehyde prior to endogenous alkaline phosphatase activity to visualize the blood vessels. (A) Vehicle control embryo in DMSO. (B) Girinimbine treated embryo. (C) Positive control embryo treated with SU5416. White arrows denote inhibited ISVs.

Abbreviations: hpf, hours post fertilization; DMSO, dimethyl sulfoxide; ISVs, inter-segmental vessels.