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. 2015 Mar 10;3:25. doi: 10.3389/fbioe.2015.00025

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Copyright © 2015 Wang, Brouillette, Ayati and Martin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The concentration of EPO as a function of “positive cells.” Each scale bar is 100 μ. Immunohistochemistry images were analyzed for “positive cells,” indicating that they were above the background thresholding technique and within our cell size criteria. (A) shows concentration at day 1 at the impact site, (B) at day 7 at the impact site, (C) at day 14 at the impact site, (D) at day 1 a distance 2 mm from the impact site, (E) at day 7 at 2 mm away, and (F) at day 14 at 2 mm away. We see clearly EPO being less constitutively expressed at day 1, when compared to day 7 or 14.