Table 4.
New Cell Phenotypes in Monkeys With Long Survivals After Multiple BrdU Injections
| A. | |||
|---|---|---|---|
| Cell type or combination | Lower | Estimate | Upper |
| All NG2%BrdU | 83% | 85% | 87% |
| PerElu | 24% | 27% | 30% |
| Pldv | 20% | 23% | 26% |
| Pld | 25% | 27% | 30% |
| NG2+ unknown | 5% | 7% | 8% |
| NG2− perivascular | 3% | 4% | 5% |
| NG2− NOT perivascular | 9% | 11% | 13% |
| NG2+ perivascular combined | 48% | 51% | 54% |
| All perivascular | 51% | 54% | 57% |
| Multibranched | 48% | 51% | 54% |
| NOT Multibranched | 31% | 34% | 37% |
| B. | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| NG2+ cells | NG2− cells | Combined cell types | |||||||||
| Group (BrdU#) | Per-Elu | Pldv | Pld | NG2+ unk | perivasc. | NOT perivasc. | Total NG2+ | All NG2+ perivasc. | All perivasc. | NG2+ Multi-branch | NG2+ NOT multi-branch |
| OC (136) | 26 ± 8 (35) | 39 ± 10 (53) | 21 ± 8 (28) | 6 ± 1 (8) | 3 ± 2 (4) | 6 ± 3 (8) | 91 ± 3 (124) | 65 ± 6 | 68 ± 7 | 60 ± 11 | 32 ± 8 |
| OR (185) | 34 ± 4 (62) | 19 ± 2 (35) | 25 ± 4 (46) | 8 ± 4 (14) | 3 ± 2 (6) | 12 ± 5 (22) | 85 ± 3 (157) | 52 ± 5 | 56 ± 4 | 44 ± 3 | 41 ± 3 |
| ORS (154) | 32 ± 6 (50) | 21 ± 7 (32) | 21 ± 13 (33) | 10 ± 4 (16) | 5 ± 1 (8) | 10 ± 4 (15) | 85 ± 3 (131) | 53 ± 2 | 58 ± 3 | 42 ± 13 | 43 ± 9 |
| YC (84) | 30 ± 12 (25) | 19 ± 8 (16) | 32 ± 10 (27) | 2 ± 1 (2) | 5 ± 4 (4) | 12 ± 3 (10) | 83 ± 2 (70) | 49 ± 3 | 54 ± 8 | 51 ± 14 | 32 ± 14 |
| YR (204) | 21 ± 3 (42) | 30 ± 5 (61) | 33 ± 6 (68) | 5 ± 2 (10) | 4 ± 2 (8) | 7 ± 2 (15) | 89 ± 3 (181) | 50 ± 3 | 54 ± 2 | 50 ± 10 | 25 ± 6 |
| YRS (376) | 24 ± 5 (92) | 13 ± 3 (48) | 31 ± 3 (115) | 10 ± 2 (36) | 3 ± 2 (10) | 20 ± 4 (75) | 77 ± 4 (291) | 37 ± 6 | 40 ± 6 | 37 ± 3 | 34 ± 6 |
| Comparisons | P values | ||||||||||
| OC - OR | ** | * | – | ||||||||
| OC - ORS | * | * | |||||||||
| OC - YC | * | ||||||||||
| OC - YRS | ** | ** | ** | ** | ** | * | – | ||||
| OR - YR | ** | – | |||||||||
| OR - YRS | * | ||||||||||
| ORS – YR | ** | ||||||||||
| YR - YRS | * | * | * | ** | – | ||||||
| YRS – ORS | ** | ** | |||||||||
| All Run-stops v All Controls | ** | * | * | * | ** | ** | ** | – | |||
| All Runners v All Controls | – | ||||||||||
| All Runners v All Run-stops | ** | * | * | * | ** | – | |||||
A: Estimated mean percentages and ranges for new cell phenotypes and cell combinations based on binomial regression modeling. B: Above, mean percentages of BrdU+ cell totals and SEMs for NG2+ and NG2− cell types and cell combinations from each group of experimental exercise monkeys. Cell counts are in parentheses and italicized. Below, P values of significant differences in paired comparisons of exercise groups. Calculated P-values correspond to comparing the log odds ratios resulting from binomial regression analysis.
P = 0.05 −0.01
P > 0.01. OC, older control group; OR, older runner group; ORS, older run-stop group; YC, younger control group; YR, younger runner group; YRS, younger run-stop group; n = 4 except YC where n = 3.