Skip to main content
. 2014 Dec 22;(94):52117. doi: 10.3791/52117
Sample CF1-D CF1-F CF4-B CF4-C
Treatment None Ribo-Zero None Ribo-Zero None Ribo-Zero None Ribo-Zero
Preprocessed reads 2,088 1,991 40,876 25,238 19,728 32,737 31,791 36,172
Mean read length 275 245 262 270 233 259 240 267
Total rRNA reads 1,737 91 29,499 17,267 5,285 291 16,371 1,761
83.20% 4.60% 72.20% 68.40% 26.80% 0.90% 51.50% 4.90%
Microbial rRNA 1,414 32 19,978 12,035 23 227 6,916 1,076
67.70% 1.60% 48.90% 47.70% 0.10% 0.70% 21.80% 3.00%
Eukaryota rRNA 323 59 9,520 5,232 5,262 64 9,455 683
15.50% 3.00% 23.30% 20.70% 26.70% 0.20% 29.70% 1.90%
% rRNA removed* 0% 95% 0% 5% 0% 97% 0% 91%
Non-rRNA reads 351 (16.8%) 1,900 (95.4%) 11,377 (27.8%) 7,971 (31.6%) 14,443 (73.2%) 32,446 (99.1%) 15,420 (48.5%) 34,411 (95.1%)
Total NR hits 102 (4.9%) 691 (34.7%) 3,327 (8.1%) 2,857 (11.3%) 4,938 (25.0%) 10,751 (32.8%) 5,905 (18.6%) 15,766 (43.6%)
Eukaryotic 74 407 2,790 2,524 4,614 10,227 4,553 8,274
Bacterial 26 283 520 312 287 471 1,326 7,442
Unassigned reads 249 (11.9%) 1,209 (60.7%) 8,050 (19.7%) 5,114 (20.3%) 9,505 (48.2%) 21,695 (66.3%) 9,515 (29.9%) 18,645 (51.5%)
*The amount of rRNA removed expressed as a percentage of the amount present in the non-depleted aliquot.