Sample | CF1-D | CF1-F | CF4-B | CF4-C | ||||
Treatment | None | Ribo-Zero | None | Ribo-Zero | None | Ribo-Zero | None | Ribo-Zero |
Preprocessed reads | 2,088 | 1,991 | 40,876 | 25,238 | 19,728 | 32,737 | 31,791 | 36,172 |
Mean read length | 275 | 245 | 262 | 270 | 233 | 259 | 240 | 267 |
Total rRNA reads | 1,737 | 91 | 29,499 | 17,267 | 5,285 | 291 | 16,371 | 1,761 |
83.20% | 4.60% | 72.20% | 68.40% | 26.80% | 0.90% | 51.50% | 4.90% | |
Microbial rRNA | 1,414 | 32 | 19,978 | 12,035 | 23 | 227 | 6,916 | 1,076 |
67.70% | 1.60% | 48.90% | 47.70% | 0.10% | 0.70% | 21.80% | 3.00% | |
Eukaryota rRNA | 323 | 59 | 9,520 | 5,232 | 5,262 | 64 | 9,455 | 683 |
15.50% | 3.00% | 23.30% | 20.70% | 26.70% | 0.20% | 29.70% | 1.90% | |
% rRNA removed* | 0% | 95% | 0% | 5% | 0% | 97% | 0% | 91% |
Non-rRNA reads | 351 (16.8%) | 1,900 (95.4%) | 11,377 (27.8%) | 7,971 (31.6%) | 14,443 (73.2%) | 32,446 (99.1%) | 15,420 (48.5%) | 34,411 (95.1%) |
Total NR hits | 102 (4.9%) | 691 (34.7%) | 3,327 (8.1%) | 2,857 (11.3%) | 4,938 (25.0%) | 10,751 (32.8%) | 5,905 (18.6%) | 15,766 (43.6%) |
Eukaryotic | 74 | 407 | 2,790 | 2,524 | 4,614 | 10,227 | 4,553 | 8,274 |
Bacterial | 26 | 283 | 520 | 312 | 287 | 471 | 1,326 | 7,442 |
Unassigned reads | 249 (11.9%) | 1,209 (60.7%) | 8,050 (19.7%) | 5,114 (20.3%) | 9,505 (48.2%) | 21,695 (66.3%) | 9,515 (29.9%) | 18,645 (51.5%) |
*The amount of rRNA removed expressed as a percentage of the amount present in the non-depleted aliquot. |