Study | Muscle | number of mice | AChR area | Synaptophysin area | Overlap area |
mean ± SD (μm2) | mean ± SD (μm2) | mean ± SD (μm2) | |||
Morsch et al. (2012)1 | gastrocnemius | 3 | 181 ± 7 | 163 ± 24 | 109 ± 29 |
diaphragm | 3 | 130 ± 41 | 102 ± 21 | 76 ± 26 | |
Morsch et al. (2013)1 | tibialis anterior | 3 | 166 ± 26 | 117 ± 21 | n.d. |
Cheng et al. (2013)2 | tibialis anterior | 4 | 226 ± 18 | 150 ± 44 | 110 ± 34 |
Tse (unpublished)2 | tibialis anterior | 7 | 213 ± 27 | 157 ± 23 | 111 ± 11 |
1 Imaged on a Zeiss LSM 510 Meta microscope but with fixed gain and offset levels. Thresholding and synaptic areas measurements used Metamorph software by MM. | |||||
2 Imaged on a Leica DM IRE2 microscope and analyzed following current protocol by the indicated first author, blind to treatment group. | |||||
n.d. not determined. |