Name	Amount
Dig-NTP Mix	5 µl of 20 mM CTP
(40 µl reaction)	5 µl of 20 mM GTP
	5 µl of 20 mM ATP
	3.25 µl of 20mM UTP
	3.5 µl of 10mM Dig-11-UTP
	18.25 µl distilled, autoclaved water
Probe Synthesis	x µl of template DNA (dependent on concentration)
(20 µl reaction)	x µl of distilled, autoclaved water
	4 µl of Dig-NTP mix
	0.5 µl of RNAse inhibitor
	2 µl of 10X RNA polymerase buffer
	2 µl of RNA polymerase