Name of the Reagent	Approximate Volume	Duration	Temperature
100% Methanol	2 ml	5 min, rocking	Room Temperature
75% Methanol	2 ml	5 min, rocking	Room Temperature
50% Methanol	2 ml	5 min, rocking	Room Temperature
25% Methanol	2 ml	5 min, rocking	Room Temperature
TTw	2 ml	10 min, rocking	Room Temperature
TTw	2 ml	10 min, rocking	Room Temperature
TTw	2 ml	10 min, rocking	Room Temperature
Pre-warm RNA Hybridization Buffer and Probe to 65°C
TTw	4 ml	5 min, rocking	Room Temperature
TTw	4 ml	5 min, rocking	Room Temperature
RNA Hybridization Buffer	2 ml	10 min, rocking	Room Temperature
Pre-warmed RNA Hybridization Buffer	2 ml	1 hr, rocking	65°C
Probe Solution	1 ml	Overnight, rocking	65°C