Name of the Reagent	Approximate Volume	Duration	Temperature
Pre-warm RNA Hybridization Buffer and 02.x SSC to 65°C and 2x SSC to 37°C
Return probe solution to tube for repeat use
RNA Hybridization Buffer	2 ml	10 min, rocking	65°C
2X SSC	2 ml	20 min, rocking	37°C
2X SSC	2 ml	20 min, rocking	37°C
0.2x SSC	4 ml	1 hr, rocking	65°C
0.2x SSC	4 ml	1 hr, rocking	65°C
MAB+HTSS+BR	1.5 ml	30 min, rocking	Room Temperature
MAB+HTSS+BR+anti-DIG antibody	1.5 ml	Overnight, rocking	4°C