Name of the Reagent	Approximate Volume	Duration	Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
MAB	4 ml	30 min, rocking	Room Temperature
BM Purple AP Substrate	500-750 µl	Overnight, rocking (see text)	Room Temperature/37 °C (see text)