Name of the Reagent	Approximate Volume	Duration	Temperature
25% Methanol	2 ml	5 min, rocking	Room Temperature
50% Methanol	2 ml	5 min, rocking	Room Temperature
75% Methanol	2 ml	5 min, rocking	Room Temperature
100% Methanol (cold)	2 ml	20 min, rocking	Room Temperature
100% Methanol (cold)	2 ml	Varies, no rocking	Room Temperature
75% Methanol	2 ml	5 min, rocking	Room Temperature
50% Methanol	2 ml	5 min, rocking	Room Temperature
25% Methanol	2 ml	5 min, rocking	Room Temperature
Mempfa	2 ml	30 min, rocking	Room Temperature
25% Methanol	2 ml	5 min, rocking	Room Temperature
25% Methanol	2 ml	5 min, rocking	Room Temperature
25% Methanol	2 ml	5 min, rocking	Room Temperature
Bleaching Solution	4 ml	40 min-3 hr, rocking	Room Temperature/37 °C (see text)
Storing Embryos
25% Methanol	2 ml	5 min, rocking	Room Temperature
50% Methanol	2 ml	5 min, rocking	Room Temperature
75% Methanol	2 ml	5 min, rocking	Room Temperature
100% Methanol	4 ml		Store at -20 °C
Imaging Embryos
1x PBS	2 ml	5 min, rocking	Room Temperature
1x PBS	2 ml	5 min, rocking	Room Temperature
1x PBS	2 ml	5 min, rocking	Room Temperature