Name	Final Concentration	Amount/100 ml
Mempfa	1 mM MgSO4	100 µl of 1 M MgSO4
(store at 4°C)	2 mM EGTA, pH 8.0	10 ml of 20 mM EGTA, pH 8.0
	0.1 M MOPS, pH 7.5	10 ml of 1 M MOPS, pH 7.5
	4% Paraformaldehyde, pH 7.5	50 ml of 8% Paraformaldehyde, pH 7.5
TTw	50 mM Tris, pH 7.4	5 ml of 1 M Tris, pH 7.4
	200 mM NaCl	4 ml of 5 M NaCl
	0.1% Tween 20	100 µl of Tween 20
100X Denhardt's Solution	2 % BSA	2 g BSA
(filter through 0.2 µm filter,	2% PVP-40	2 g PVP-40
store at -20°C)	2% Ficoll 400	2 g Ficoll 400
20X SSC	3 M NaCl	17.5 g NaCl
	300 mM Trisodium Citrate	8.8 g Trisodium Citrate
RNA Hybridization Buffer	50% formamide	50 ml of 100% formamide
(store at 4°C)	5x SSC	25 ml of 20x SSC
	1 mg/ml yeast RNA	4 ml of 1mg/ml yeast RNA dissolved in 50% formamide
	1X Denhardt's Solution	1 ml of 100x Denhardt's Solution
	0.1% Tween 20	100 µl of Tween 20
	5 mM EDTA, pH 8.0	1 ml of 0.5 M EDTA, pH 8.0
MAB	100 mM Maleic Acid	1.16 g of Maleic Acid
(pH 7.5, store at 4°C)	150 mM NaCl	0.88 g of NaCl
MAB+HTSS+BR	100 mM Maleic Acid	96 ml of MAB
	4% Heat Treated Sheep Serum	4 ml of Heat Treated Sheep Serum (heat treated at 55°C for 30 min and aliquoted)
	2% Blocking Reagent	2 g of Roche Blocking Reagent
MAB+HTSS+BR+anti-Dig antibody	100 mM Maleic Acid	96 ml of MAB
	4% Heat Treated Sheep Serum	4 ml of Heat Treated Sheep Serum
	2% Blocking Reagent	2 g of Roche Blocking Reagent
	1:10,000 Anti-Dig-AP, Fab Fragments Antibody	1.5 µl of Anti-Digoxygenin-AP, Fab Fragments Antibody
Alkaline Phosphatase (AP) Buffer	100 mM Tris, pH 9.5	10 ml of 1 M Tris, pH 9.5
	50 mM MgCl2	5 ml of 1 M MgCl2
	100 mM Nacl	2.5 ml of 4 M NaCl
	0.1% Tween 20	100 µl of Tween 20
Bleaching Solution	0.3% H2O2	3.34 ml of 30% H2O2
	5% Formamide	5 ml of 100% Formamide
	0.5% SSC	2.5 ml of 20x SSC
Clearing Solution	1/3 Benzyl Alcohol	33 ml
	2/3 Benzyl Benzoate	67 ml