Medium composition	Concentration	Catalogue no.	Company
Preadipocyte Growth medium		C-27410 (ready to use)
Foetal Calf Serum	0.05 ml/ml		PromoCell
Endothelial Cell Growth supplement	0.004 ml/ml		PromoCell
Epidermal Growth Factor (recombinant human)	10 ng/ml		PromoCell
Penicillin	100 U/ml		Sigma
Streptomycin	100 μg/ml		Sigma
Glutamine	292 μg/ml		Sigma
D-Biotin	8.0 μg/ml		PromoCell
Insulin (recombinant human)	0.5 μg/ml		PromoCell
Dexamethasone	400 ng/ml		PromoCell
IBMX	44 μg/ml		PromoCell
L-Thyroxine	9 ng/ml		PromoCell
Ciglitazone	3 μg/ml		PromoCell
Penicillin	100 U/ml		Sigma
Streptomycin	100 μg/ml		Sigma
Glutamine	292 μg/ml		Sigma
Preadipocyte differentiation medium		C-27436 (ready to use)
D-Biotin	8.0 μg/ml		PromoCell
Insulin (recombinant human)	0.5 μg/ml		PromoCell
Dexamethasone	400 ng/ml		PromoCell
IBMX	44 μg/ml		PromoCell
L-Thyroxine	9 ng/ml		PromoCell
Ciglitazone	3 μg/ml		PromoCell
Penicillin	100 U/ml		Sigma
Streptomycin	100 μg/ml		Sigma
Glutamine	292 μg/ml		Sigma
Adipocyte nutrition medium		C-27438 (ready to use)
D-Biotin	8.0 μg/ml		PromoCell
Insulin (recombinant human)	0.5 μg/ml		PromoCell
Dexamethasone	400 ng/ml		PromoCell
Penicillin	100 U/ml		Sigma
Streptomycin	100 μg/ml		Sigma
Glutamine	292 μg/ml		Sigma
Note: PromoCell, Heidelberg, Germany; Sigma-Aldrich Company Ltd, Dorset, UK