| Medium components | Concentration | Catalogue no. | Commercial source |
| Human skeletal muscle growth medium: | C-23060 (Comes 'ready to use' containing all factors listed) | ||
| Skeletal muscle basal medium | - | PromoCell | |
| Foetal Calf Serum | 15% | PromoCell (5%) & FCS Gold, PAA Laboratories (10%) - Cat no. A15-151 | |
| Fetuin (bovine) | 50 μg/ml | PromoCell | |
| Epidermal Growth factor | 10 ng/ml | PromoCell | |
| Basic fibroblast growth factor (Recombinant human) | 1 ng/ml | PromoCell | |
| Dexamethasone | 0.4 μg/ml | PromoCell | |
| Insulin (recombinant human) | 10 μg/ml | PromoCell | |
| Penicillin | 100 U/ml | Sigma | |
| Streptomycin | 100 μg/ml | Sigma | |
| L-Glutamine | 292 μg/ml | Sigma | |
| Myogenic differentiation medium | C-23260 | ||
| Skeletal muscle basal medium | - | PromoCell | |
| Penicillin | 100 U/ml | Sigma | |
| Streptomycin | 100 μg/ml | Sigma | |
| Note: PromoCell, Heidelberg, Germany; Sigma-Aldrich Company Ltd, Dorset, UK |