Medium components
|
Concentration
|
Catalogue no.
|
Commercial source
|
Human skeletal muscle growth medium: |
|
C-23060 (Comes 'ready to use' containing all factors listed) |
|
Skeletal muscle basal medium |
- |
|
PromoCell |
Foetal Calf Serum |
15% |
|
PromoCell (5%) & FCS Gold, PAA Laboratories (10%) - Cat no. A15-151 |
Fetuin (bovine) |
50 μg/ml |
|
PromoCell |
Epidermal Growth factor |
10 ng/ml |
|
PromoCell |
Basic fibroblast growth factor (Recombinant human) |
1 ng/ml |
|
PromoCell |
Dexamethasone |
0.4 μg/ml |
|
PromoCell |
Insulin (recombinant human) |
10 μg/ml |
|
PromoCell |
Penicillin |
100 U/ml |
|
Sigma |
Streptomycin |
100 μg/ml |
|
Sigma |
L-Glutamine |
292 μg/ml |
|
Sigma |
Myogenic differentiation medium |
|
C-23260 |
|
Skeletal muscle basal medium |
- |
|
PromoCell |
Penicillin |
100 U/ml |
|
Sigma |
Streptomycin |
100 μg/ml |
|
Sigma |
Note: PromoCell, Heidelberg, Germany; Sigma-Aldrich Company Ltd, Dorset, UK |
|