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. 2015 Jan 12;(95):52049. doi: 10.3791/52049
Medium components Concentration Catalogue no. Commercial source
Human skeletal muscle growth medium: C-23060 (Comes 'ready to use' containing all factors listed)
Skeletal muscle basal medium - PromoCell
Foetal Calf Serum 15% PromoCell (5%) & FCS Gold, PAA Laboratories (10%) - Cat no. A15-151
Fetuin (bovine) 50 μg/ml PromoCell
Epidermal Growth factor 10 ng/ml PromoCell
Basic fibroblast growth factor (Recombinant human) 1 ng/ml PromoCell
Dexamethasone 0.4 μg/ml PromoCell
Insulin (recombinant human) 10 μg/ml PromoCell
Penicillin 100 U/ml Sigma
Streptomycin 100 μg/ml Sigma
L-Glutamine 292 μg/ml Sigma
Myogenic differentiation medium C-23260
Skeletal muscle basal medium - PromoCell
Penicillin 100 U/ml Sigma
Streptomycin 100 μg/ml Sigma
Note: PromoCell, Heidelberg, Germany; Sigma-Aldrich Company Ltd, Dorset, UK