PCR MASTER-MIX SET-UP
Component	µl per reaction
Water	19.45
10x Taq buffer	2.5
dNTPs (10 mM stock)	0.675
DMSO	0.75
Taq enzyme	0.125
Forward Primer (100 uM stock)	0.25
Reverse primer (100 uM stock)	0.25
Heavy Chain Sequencing Primers
M13 Forward primer	GTAAAACGACGGCCAGTACTCGAGGCTGAGCAAAGC
M13 Reverse primer	CAGGAAACAGCTATGACGGGAAGTGTCCTTGACCA
Light Chain Sequencing Primers
M13 Forward primer	TGTAAAACGACGGCCAGTCTGTCATAAAGTTGTCACGG
M13 Reverse primer	CAGGAAACAGCTATGACCCCTTGGTACCCTGTCCG
PCR SETTINGS
Step 1.	94 °C for 3:00 min
Step 2.	94 °C for 0:30 min
Step 3.	55 °C for 0:30 min
Step 4.	72 °C for 1:00 min
Return to Step 2 and repeat 24x	jump to step 2
Step 5.	72°C for 7:00 min
Step 6.	12 °C hold