SOLUTIONS | ||
2YT media - Add water to 1 L, adjust pH to 7.0, and autoclave. | yeast extract | 10 g |
tryptone | 16 g | |
NaCl | 5 g | |
NZY media (1 L) | N-Z Amine | 10 g |
yeast extract | 5 g | |
50X ZYM-5052 | 20 ml | |
20 salt stock | 50 ml | |
50x ZYM-5052 sugar stock (1 L) | glucose | 25 g |
lactose | 100 g | |
glycerol | 250 ml | |
20x salt stock (1 L) | Na2HPO4 | 500 mM |
KH2PO4 | 500 mM | |
NH4Cl | 1 M | |
Na2SO4 | 100 mM | |
Carbenicillin (Carb): | 100 mg/ml in water. Filter-sterilize. | |
Kanamycin (Kan): | 25 mg/ml in water. Filter-sterilize. | |
Tetracycline (Tet): | 10 mg/ml in water. Filter sterilize. | |
PEG-NaCl | PEG | 20% |
NaCl | 2.5 M | |
1x PBS (pH7.2) | NaCl | 137 mM |
KCl | 3 mM | |
Na2HPO4 | 8 mM | |
KH2PO4 | 1.5 mM | |
1X KCM | KCl | 500 mM |
CaCl2 | 150 mM | |
MgCl2 | 250 mM | |
SOC Media | yeast extract | 5 g/L |
tryptone | 20 g/L | |
NaCl | 10 mM | |
KCl | 2.5 mM | |
MgSO4 | 20 mM | |
Glucose | 20 mM | |
Blocking buffer | PBS | 1x |
BSA | 0.20% | |
PBT buffer | PBS | 1x |
BSA | 0.20% | |
Tween | 0.05% | |
PT buffer | PBS | 1x |
Tween | 0.05% | |
Phosphoric acid | H3P04 | 1 M |