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. 2015 Jan 17;(95):51492. doi: 10.3791/51492
SOLUTIONS
2YT media  - Add water to 1 L, adjust pH to 7.0, and autoclave. yeast extract  10 g
tryptone 16 g
NaCl 5 g
NZY media (1 L) N-Z Amine 10 g
yeast extract  5 g
50X ZYM-5052 20 ml
20 salt stock 50 ml
50x ZYM-5052 sugar stock (1 L) glucose 25 g
lactose 100 g
glycerol 250 ml
20x salt stock (1 L) Na2HPO4 500 mM
KH2PO4 500 mM
NH4Cl 1 M
Na2SO4 100 mM
Carbenicillin (Carb):  100 mg/ml in water. Filter-sterilize.
Kanamycin (Kan):  25 mg/ml in water. Filter-sterilize.
Tetracycline (Tet):  10 mg/ml in water. Filter sterilize.
PEG-NaCl PEG 20%
NaCl 2.5 M
 1x PBS (pH7.2)  NaCl  137 mM
KCl 3 mM
Na2HPO4 8 mM
KH2PO4 1.5 mM
1X KCM  KCl 500 mM
CaCl2 150 mM
MgCl2 250 mM
SOC Media yeast extract 5 g/L
tryptone 20 g/L
NaCl 10 mM
KCl 2.5 mM
MgSO4 20 mM 
Glucose 20 mM 
Blocking buffer  PBS 1x
BSA 0.20%
PBT buffer PBS 1x
BSA 0.20%
Tween 0.05%
PT buffer PBS 1x
Tween 0.05%
Phosphoric acid H3P04 1 M