| Concentration in bath | Addition of agonist made to bath |
| 1 x 10-9 M | 50 ml 1 x 10-6 M |
| 3 x 10-9 M | 100 ml 1 x 10-6 M |
| 1 x 10-8 M | 35 ml 1 x 10-5 M |
| 3 x 10-8 M | 100 ml 1 x 10-5 |
| 1 x 10-7 M | 35 ml 1 x 10-4 M |
| 3 x 10-7 M | 100 ml 1 x 10-4 M |
| 1 x 10-6 M | 35 ml 1 x 10-3 M |
| 3 x 10-6 M | 100 ml 1 x 10-3 M |
| 1 x 10-5 M | 35 ml 1 x 10-2 M |
| 3 x 10-5 M | 100 ml 1 x 10-2 M |
| 1 x 10-4 M | 35 ml 1 x 10-1 M |
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