Fig. 5.

Cut-off point searching in the histogram of the b1 image. (A) the histogram of b1 image generated from the image shown in Fig. 6(A). The numbers and the b1 values of background pixels are more than the Hematoxylin-stained pixels, so the background pixels form a peak in the histogram. For the stains diffusing and heterogeneity, the Hematoxylin-stained pixels distribute in the low value zones; (B) the spectrum displaying the curve of frequency distribution of ten classes image. The b1 values of the Hematoxylin-stained pixels range from 0 to 60, so the point before the peak is set as a cut-off.