Fig. 10.

(a) Comparing widefield fluorescence, MP intensity and fluorescence lifetime data sets of control MCF-7 cells expressing EGFR-EGFP vs test MCF-7 cells expressing EGFR-EGFP & Grb2-mCherry, pre and post treated with EGF ligand. Acquisition time per frame is 10 seconds and all images have an 87.5µm x 87.5µm field of view.(b) Statistical analysis comparing control MCF-7 cells expressing EGFR-EGFP with MCF-7 cells coexpressing EGFR-EGFP & Grb2-mCherry, both pre and post addition of EGF ligand. In order to compare two different populations (control vs. test) for significance two-tailed unpaired Student t-test was used. For the same populations (pre vs. post treatment) paired t-test was used. *** between populations denotes a highly significant difference in lifetime values (p<0.0001).