| Chappel-Perry buffer I (CPI) | |
| Chemical | Concentration |
| KCl | 100 mM |
| MOPS | 50 mM |
| EDTA | 1 mM |
| MgSO4 | 5 mM |
| ATP | 1 mM |
| pH | 7.4 |
| Chappel-Perry buffer II (CPII) | |
| Chemical | Concentration |
| KCl | 100 mM |
| MOPS | 50 mM |
| EDTA | 1 mM |
| MgSO4 | 5 mM |
| ATP | 0.2 mM |
| Fatty-acid free BSA | 0.50% |
| pH | 7.4 |
| Mitochondrial Assay Solution (MAS) (2X) | |
| Chemical | Concentration |
| Sucrose | 35 mM |
| Mannitol | 110 mM |
| KH2PO4 | 2.5 mM |
| MgCl2 | 2.5 mM |
| HEPES | 1.0 mM |
| EGTA | 0.5 mM |
| Fatty-acid free BSA | 0.10% |
| pH | 7.4 |
| Mitochondrial Assay Solution (MAS) with complex II initial conditions | |
| Chemical | Concentration |
| 1X MAS | |
| Succinate | 10 mM |
| Rotenone | 2 μM |
| pH | 7.4 |
| Mitochondrial Assay Solution (MAS) with complex I initial conditions | |
| Chemical | Concentration |
| 1X MAS | |
| Pyruvate | 5 mM |
| Malate | 5 mM |
| pH | 7.4 |
| *All buffers to be made in deionized water |
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