Stock concn	Final reaction concn	Volume per reaction(µl)
DDW	-	-	10.1
ThermoPol buffer	10 x	1 x	2.5
MgSO4	100 mM	2 mM	0.5
Primer mix*	Table 2	2.5
dNTPs	25 mM	400 uM	0.4
Betaine	5 M	1 M	5
Hydroxynaphthol blue	3 mM	120 µM	1
Bst DNA polymerase	8,000 U/ml	8 U	1
Total			23