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. 2015 Mar 2;10:1667–1678. doi: 10.2147/IJN.S77527

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© 2015 Luo et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Optimization of the incubation time for transfection. Cell line: Hep G2. Plate: six-well format. Complex: polyethyleneimine (PEI)/pEGFP-C1. Standard: standard transfection. 0.5–4.0 hours: different incubation time in the modified transfection cases with 700 μL transfection complex. (A) Transfection efficiency analyzed by fluorescence-activated cell sorting 48 hours after transfection. (B) Comparison of the transfection efficiency.

Note: *P<0.05 versus others except 3.0 hours (1.5 hours vs 3.0 hours: P>0.05).