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. 2015 Mar 2;10:1667–1678. doi: 10.2147/IJN.S77527

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© 2015 Luo et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Cell viability assay in different transfection cases. (A) and (B) The influence of incubation time on cell viability in polyethyleneimine (PEI)- (A) and Lipofectamine™ 2000 (Lipo2000)-mediated (B) transfection cases. Cell line: Hep G2. Plate: 24-well format. Time: 48 hours after transfection. Standard: standard transfection. Modified: modified transfection (300 μL/well transfection complex and 1.5 hours’ incubation). 0.5–4.0 hours: different incubation time in modified transfection cases with 300 μL/well transfection complex (P<0.05 vs others). (C) Comparison of the cell viability in different transfection methods. PEI- and Lipo-M: PEI- and Lipo2000-modified transfection; PEI- and Lipo-S: PEI and Lipo2000 standard transfection; PEI- and Lipo-S2: PEI and Lipo2000 standard transfection with increased complex concentration. 24, 48, and 72 hours: cell viability was analyzed 24, 48, and 72 hours after transfection (P<0.05 vs standard transfection).