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Comparison of the transfection efficiency between the standard and modified methods in different cell strains. The transfection efficiencies were measured by fluorescence-activated cell sorting (A) and (B) and luciferase activity assay (C) and (D), and presented as the relative folds by comparing the data between modified (M) and standard (S) methods. Plate: six-well format. Time: 48 hours after transfection.
Abbreviations: PEI, polyethyleneimine; Lipo2000, Lipofectamine™ 2000.
