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. 2015 Mar 2;10:1667–1678. doi: 10.2147/IJN.S77527

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© 2015 Luo et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Analysis of relative transfection efficiency after the inhibition of specific pathways by endocytosis inhibitors. Cell line: Hep G2. Plate: six-well format. S: standard transfection. M: modified transfection. Cells were treated with inhibitors for 1 hour followed with pEGFP-C1 transfection. Proportion of enhanced green fluorescent protein-expressing cells in each group was analyzed by fluorescence-activated cell sorting 48 hours later and the data were normalized to those of the untreated group.

Abbreviations: PEI, polyethyleneimine; Lipo2000, Lipofectamine™ 2000; MβCD, methyl-β-cyclodextrin.